Enhanced Osteogenic Differentiation of Stem Cells on Phase-Engineered Graphene Oxide

Jia Wei Yang, Kuan Yu Hsieh, Priyank V. Kumar, Sheng Jen Cheng, You Rong Lin, Yu Chih Shen, Guan-Yu Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Graphene oxide (GO) has attracted significant interest as a template material for multiple applications due to its two-dimensional nature and established functionalization chemistries. However, for applications toward stem cell culture and differentiation, GO is often reduced to form reduced graphene oxide, resulting in a loss of oxygen content. Here, we induce a phase transformation in GO and demonstrate its benefits for enhanced stem cell culture and differentiation while conserving the oxygen content. The transformation results in the clustering of oxygen atoms on the GO surface, which greatly improves its ability toward substance adherence and results in enhanced differentiation of human mesenchymal stem cells toward the osteogenic lineage. Moreover, the conjugating ability of modified GO strengthened, which was examined by auxiliary osteogenic growth peptide conjugation. Overall, our work demonstrates GO's potential for stem cell applications while maintaining its oxygen content, which could enable further functionalization and fabrication of novel nano-biointerfaces.

Original languageEnglish
Pages (from-to)12497-12503
Number of pages7
JournalACS Applied Materials and Interfaces
Issue number15
StatePublished - 18 Apr 2018

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