Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping

Hsu Hua Yeh, Shu Lin Chang, Yi Ming Chiang, Kenneth S. Bruno, Berl R. Oakley, Tung-Kung Wu*, Clay C.C. Wang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

We reannotated the A. niger NR-PKS gene, e-gw1-19.204, and its downstream R domain gene, est-GWPlus-C-190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID-06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

Original languageEnglish
Pages (from-to)756-759
Number of pages4
JournalOrganic Letters
Volume15
Issue number4
DOIs
StatePublished - 15 Feb 2013

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