Electrical detection of DNA hybridization with multilayer gold nanoparticles between nanogap electrodes

C. Y. Tsai, Y. H. Tsai, C. C. Pun, B. Chan, T. Y. Luh, C. C. Chen, Fu-Hsiang Ko, P. J. Chen, P. H. Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

This study presents a DNA detection method via hybridization of a probe oligonucleotide with a target DNA and with a substrate oligonucleotide, which leads to self-assembly of gold nanoparticles and a change in the observed current. In contrast to previously reported methods, the present method can be used to detect a target DNA without a silver enhancement step. The device can be washed with 0.3 M PBS below 50 °C for a few minutes, indicating the stringency of target DNA hybridization. Target DNA concentration of as low as 10 picomolar can be detected by this method. Importantly, identification of single-base-pair mismatch in the target DNA can be accomplished with this nano-gap DNA chip.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalMicrosystem Technologies
Volume11
Issue number2-3
DOIs
StatePublished - 1 Jan 2005

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