In the field of the pre-hospital emergency, immediate and medical processes must be provided to patients to increase their chance of survival. When a patient is in transit to a hospital, real-time or uninterrupted patient data shall be transmitted to the hospital so that physicians could realize the present status and make decisions of appropriate instructions and assessments. For moving ambulances, obtaining uninterrupted network services to wirelessly transmit data is the most important issue. In the proposed work, a seamless and ubiquitous stream control transmission protocol (SCTP) tunnel was developed to improve wireless communication connections. Cognitive radios can optimize spectrum usage by detecting their operating environment; therefore, it is necessary to integrate existing wireless communication services in an efficient mobile middleware (MM) and achieve a seamless transmission in a heterogeneous network. The developed efficient MM for seamless transmission, which is migrated to an embedded device, is consistent of existent network environments without requiring modification of the original system architecture. Moreover, the handoff when switching between wireless networks is shorter than that obtained in related studies. Thus, medical personnel can seamlessly transmit video and vital data to hospitals through the proposed MM while walking or in a moving vehicle.