Effects of garlic oil on the migration of neutrophil-like cell studied by using a chemotactic gradient labchip

Long Hsu*, Po Chen Shih, Chia Hao Kuo, Jenh-Yih Juang, Cheng Hsien Liu, Cheng Tzu Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We have designed and fabricated a novel chemotactic gradient Labchip for studying cell migration quantitatively. Owing to the great potential of garlic and its preparations in developing antiinflammatory drugs, the aim of the present study is to investigate the effect of garlic oil on the locomotion of a neutrophil-like cell by measuring the dynamic features of cell migration including migration direction, average migration speed, chemotactic index (CI), and motility index (MI) with the newly designed Labchip. We found that garlic oil treatment lowered the values of CI and MI and reduced the average speed of cell migration from 13 to 8 μm/min. The results indicate that garlic oil is a potential inhibitor for neutrophil-like cell migration and chemotactic responsiveness. By comparing with the effects of nocodazole and cytochalasin B, we also suggest that the antiinflammatory activity exhibited by garlic oil was mainly through inhibiting the assembly-disassembly processes of the cytoskeleton.

Original languageEnglish
Article number319059
JournalJournal of Biomedicine and Biotechnology
Volume2010
DOIs
StatePublished - 24 May 2010

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