Effects of diacetyl monoxime and cytochalasin D on ventricular fibrillation in swine right ventricles

Moon Hyoung Lee, Shien-Fong Lin, Toshihiko Ohara, Chikaya Omichi, Yuji Okuyama, Eugene Chudin, Alan Garfinkel, James N. Weiss, Hrayr S. Karagueuzian, Peng Sheng Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Whether or not the excitation-contraction (E-C) uncoupler diacetyl monoxime (DAM) and cytochalacin D (Cyto D) alter the ventricular fibrillation (VF) activation patterns is unclear. We recorded single cell action potentials and performed optical mapping in isolated perfused swine right ventricles (RV) at different concentrations of DAM and Cyto D. Increasing the concentration of DAM results in progressively shortened action potential duration (APD) measured to 90% repolarization, reduced the slope of the APD restitition curve, decreased Kolmogorov-Sinai entropy, and reduced the number of VF wave fronts. In all RVs, 15-20 mmol/l DAM converted VF to ventricular tachycardia (VT). The VF could be reinduced after the DAM was washed out. In comparison, Cyto D (10-40 μmol/l) has no effects on APD restitution curve or the dynamics of VF. The effects of DAM on VF are associated with a reduced number of wave fronts and dynamic complexities in VF. These results are compatible with the restitution hypothesis of VF and suggest that DAM may be unsuitable as an E-C uncoupler for optical mapping studies of VF in the swine RVs.

Original languageEnglish
Pages (from-to)H2689-H2696
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume280
Issue number6
DOIs
StatePublished - 3 Jul 2001

Keywords

  • Action potentials
  • Defibrillation
  • Electrophysiology
  • Reentry
  • Tachyarrhythmias

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