Effects of α and β recombinant FSH (Gonal-F, Puregon) and progesterone upon human endometrial cell proliferation in-vitro: A preliminary study

Chi Chen Chang, Yao Yuan Hsieh, Kung Hao Hsu, Chih-Sheng Lin*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Endometrial proliferation or regeneration during menstrual cycle is regulated by sexual hormones. However, the effect of gonadotrophins on the endometrial cell growth remains obscure. Herein, we aimed to investigate the effects of r-FSH (Gonal-F, Puregon) and progesterone on the proliferation of human endometrial cells in-vitro. According as gonadotrophin concentrations, the follicular-phase endometrial cells were divided into six groups: (1) 0 (controls), (2) 1; (3) 10; (4) 100; (5) 1000; (6) 100,000μIU/ml. The cell countings with microscopy and cell proliferation kit assay were used to assess the endometrial cell proliferations. In Gonal-F groups, the cell absorptions (%) after 24/48h culture were: (1) 100/100; (2) 103.8/102.3; (3) 104.8/102.8; (4) 102.3/101.3; (5) 96.3/94.2; (6) 86.8/84.3. In Puregon groups, the cell absorptions were: (1) 100/100; (2) 102.8/101.9; (3) 103/102.3; (4) 103.9/103.5; (5) 102.9/102.4; (6) 103.7/103.2 (non-different). In progesterone groups, the cell absorptions were: (1) 100/100; (2) 99.1/101.9; (3) 83.5/80.4; (4) 80.7/82.4. Higher dosage of Gonal-F (100,000μIU/ml) and progesterone (10, 100μg/ml) appeared the significant inhibition upon endometrium. We conclude that lower dosages of Gonal-F, Puregon, and progesterone appear the non-significant influence upon endometrium. Higher dosage of Gonal-F (10,000μIU/ml) and progesterone (10, 100μg/ml), but not Puregon, might interfere with the endometrial proliferation during follicular phase.

Original languageEnglish
Pages (from-to)110-116
Number of pages7
JournalGynecological Endocrinology
Volume27
Issue number2
DOIs
StatePublished - 1 Feb 2011

Keywords

  • Endometrium
  • gonadotrophin
  • Gonal-F
  • progesterone
  • Puregon

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