Effective construction of a high-capacity boronic acid layer on a quartz crystal microbalance chip for high-density antibody immobilization

Pei Heng Lin, Sheng Cih Huang, Kuang Po Chen, Bor-Ran Li*, Yaw-Kuen Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Boronic acids (BAs) provide strong potential in orientation immobilization of antibody and the modification method is crucial for efficiency optimization. A highly effective method has been developed for rapid antibody immobilization on gold electrodes through the electrodeposition of a BA–containing linker in this study. Aniline-based BA forms a condense layer while antibody could automatically immobilize on the surface of the electrode. Compare to traditional self-assembled monolayer method, the electrodeposition process dramatically reduces the modification time from days to seconds. It also enhances the immobilized efficiency from 95 to 408 (ng/cm2) with a strong preference being exhibited for shorter aniline-based linkers.

Original languageEnglish
Article number28
JournalSensors (Switzerland)
Volume19
Issue number1
DOIs
StatePublished - 1 Jan 2019

Keywords

  • Boronic acid
  • Electrodeposition
  • Orientated antibody immobilization
  • Quartz crystal microbalance

Fingerprint Dive into the research topics of 'Effective construction of a high-capacity boronic acid layer on a quartz crystal microbalance chip for high-density antibody immobilization'. Together they form a unique fingerprint.

Cite this