Effect of substrate concentration on bioleaching of metal-contaminated sediment

Shen Yi Chen, Jih-Gaw Lin

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


The remediation of metal-contaminated sediment was studied using the bioleaching process with a mixed culture of sulfur-oxidizing bacteria. The effects of substrate concentration (elemental sulfur) on sediment acidification, sulfur oxidation and metal solubilization from contaminated sediment during the bioleaching process were investigated with free-cell suspensions. Sulfur concentration greater than 0.5% (w/v) was found to be inhibitory to bacterial activity and metal solubilization from sediment. The sulfate production was well described by a substrate inhibition expression and Haldane's equation. In addition, an empirical equation related to sulfur concentration was also used to describe the metal solubilization in the bioleaching process.

Original languageEnglish
Pages (from-to)77-89
Number of pages13
JournalJournal of Hazardous Materials
Issue number1
StatePublished - 19 Mar 2001


  • Bioleaching
  • Contaminated sediment
  • Heavy metal
  • Sulfur concentration
  • Thiobacilli

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