Early development of cutaneous cancer revealed by intravital nonlinear optical microscopy

Chun Chin Wang*, Feng Chieh Li, Wei Chou Lin, Yang Fang Chen, Shean-Jen Chen, Sung Jan Lin, Chen Yuan Dong

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We performed intravital multiphoton microscopy to image and analyze normal and carcinogen treated skin tissues of nude mice in vivo. Using intravital images and the quantitative pixel to pixel ratiometric processing of multiphoton autofluorescence to second harmonic generation index (MAFSI), we can visualize the interaction between epithelial cells and extracellular matrix. We found that as the imaging depth increases, MAFSI has different distribution in normal and treated cutaneous specimens. Since the treated skin eventually became squamous cell carcinoma, our results show that the physiological changes to mouse skin en route to become cancer can be effectively tracked by multiphoton microscopy.

Original languageEnglish
Article number113702
JournalApplied Physics Letters
Volume97
Issue number11
DOIs
StatePublished - 13 Sep 2010

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    Wang, C. C., Li, F. C., Lin, W. C., Chen, Y. F., Chen, S-J., Lin, S. J., & Dong, C. Y. (2010). Early development of cutaneous cancer revealed by intravital nonlinear optical microscopy. Applied Physics Letters, 97(11), [113702]. https://doi.org/10.1063/1.3490644