Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis

Yan Ren Lin, Chao Jui Li, Shih Han Syu, Cheng Hao Wen, Waradee Buddhakosai, Han Ping Wu, Cheng Hsu Chen, Huai En Lu*, Wen-Liang Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Postcardiac arrest acidosis can decrease survival. Effective medications without adverse side effects are still not well characterized. We aimed to analyze whether early administration of glutamine could improve survival and protect cardiomyocytes from postcardiac arrest acidosis using animal and cell models. Forty Wistar rats with postcardiac arrest acidosis (blood pH < 7.2) were included. They were divided into study (500 mg/kg L-alanyl-L-glutamine, n=20) and control (normal saline, n=20) groups. Each of the rats received resuscitation. The outcomes were compared between the two groups. In addition, cardiomyocytes derived from human induced pluripotent stem cells were exposed to HBSS with different pH levels (7.3 or 6.5) or to culture medium (control). Apoptosis-related markers and beating function were analyzed. We found that the duration of survival was significantly longer in the study group (p<0.05). In addition, in pH 6.5 or pH 7.3 HBSS buffer, the expression levels of cell stress (p53) and apoptosis (caspase-3, Bcl-xL) markers were significantly lower in cardiomyocytes treated with 50 mM L-glutamine than those without L-glutamine (RT-PCR). L-glutamine also increased the beating function of cardiomyocytes, especially at the lower pH level (6.5). More importantly, glutamine decreased cardiomyocyte apoptosis and increased these cells' beating function at a low pH level.

Original languageEnglish
Article number2106342
JournalBioMed Research International
Volume2016
DOIs
StatePublished - 1 Jan 2016

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    Lin, Y. R., Li, C. J., Syu, S. H., Wen, C. H., Buddhakosai, W., Wu, H. P., Hsu Chen, C., Lu, H. E., & Chen, W-L. (2016). Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis. BioMed Research International, 2016, [2106342]. https://doi.org/10.1155/2016/2106342