We formalize a theory to help explore the effect of conformational fluctuation on the energy landscape evolution of single-molecule protein. Using this formalization, we investigate the photon emission from single photoactivated fluorescent protein. A bimodal regulation on the energy landscape evolution was discovered, and its origin was attributed to slow conformational fluctuations of the protein matrix.
|Journal||Physical Review E - Statistical, Nonlinear, and Soft Matter Physics|
|State||Published - 31 Aug 2012|