Dormancy-break and germination in seeds of Prunus campanulata (Rosaceae): Role of covering layers and changes in concentration of abscisic acid and gibberellins

Shun Ying Chen, Ching Te Chien*, Jeng Der Chung, Yuh-Shyong Yang, Shing Rong Kuo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Intact seeds (seed+endocarp) from freshly harvested fruits of Prunus campanulata were dormant, and required 4-6 weeks of warm followed by 8 weeks of cold stratification for maximum germination percentage. Removing both endocarp and seed coat, however, promoted germination in a high percentage of non-stratified seeds. Treatment of intact, non-stratified seeds with gibberellic acid (GA3) was only partially effective in breaking dormancy. However, GA3 promoted germination of non-stratified seeds in which the endocarp (but not the seed coat) had been removed. The order of abscisic acid (ABA) concentration in fresh seeds was endocarp > seed coat > embryo, and its concentration in endocarp plus seed coat was about 6.2-fold higher than that in the embryo. Total ABA contents of seeds subjected to warm and/or cold moist stratification were reduced 6- to 12-fold. A higher concentration of GA4 was detected in embryos of non-dormant than in those of dormant seeds. Fluridone, a carotenoid biosynthesis inhibitor, was efficient in breaking dormancy of Prunus seeds. Paclobutrazol, a GA biosynthesis inhibitor, completely inhibited seed germination, and the inhibitory effect could be partially reversed by GA4, but not by GA3. Thus, dormancy in P. campanulata seeds is imposed by the covering layers. Dormancy break is accompanied by a decrease in ABA content of the covering layers and germination by an increase of embryonic GA4 content.

Original languageEnglish
Pages (from-to)21-32
Number of pages12
JournalSeed Science Research
Volume17
Issue number1
DOIs
StatePublished - 1 Mar 2007

Keywords

  • Abscisic acid
  • Cold and warm stratification
  • Endocarp
  • Fluridone
  • Germination
  • Gibberellins
  • Paclobutrazol
  • Prunus campanulata
  • Seed coat
  • Seed dormancy

Fingerprint Dive into the research topics of 'Dormancy-break and germination in seeds of Prunus campanulata (Rosaceae): Role of covering layers and changes in concentration of abscisic acid and gibberellins'. Together they form a unique fingerprint.

Cite this