Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films

Fabio Palumbo*, Giuseppe Camporeale, Yi Wei Yang, Jong-Shinn Wu, Eloisa Sardella, Giorgio Dilecce, Cosima Damiana Calvano, Laura Quintieri, Leonardo Caputo, Federico Baruzzi, Pietro Favia

*Corresponding author for this work

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 μg/cm2 as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.

Original languageEnglish
Pages (from-to)1302-1310
Number of pages9
JournalPlasma Processes and Polymers
Volume12
Issue number11
DOIs
StatePublished - 1 Nov 2015

Keywords

  • aerosol assisted plasma
  • bio-composite coating
  • dielectric barrier discharges
  • lysozyme
  • matrix-assisted laser desorption/ionization mass spectrometry

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