Direct observation of single molecule conformational change of tight-turn paperclip DNA triplex in solution

Ching Ping Liu, Ming Tsai Wey, Chia-Ching Chang*, Lou Sing Kan

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

DNA triplex modulates gene expression by forming stable conformation in physiological condition. However, it is not feasible to observe this unique molecular structure of large molecule with 54 oligodeoxynucleotides directly by conventional nuclear magnetic approach. In this study, we observed directly single molecular images of paperclip DNA triplexes formation in a buffer solution of pH 6.0 by atomic force microscopy (AFM). Meanwhile, a diffuse "tail" of unwound DNA was observed in pH 8.0 solution. This designable approach in visualizing the overall structures and shapes of oligo-DNAs at the single molecular level, by AFM, is applicable to other biopolymers as well.

Original languageEnglish
Pages (from-to)261-269
Number of pages9
JournalApplied Biochemistry and Biotechnology
Volume159
Issue number1
DOIs
StatePublished - 1 Oct 2009

Keywords

  • Atomic force microscopy
  • DNA triplex
  • Single molecule image
  • Tight-turn paperclip DNA

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