Direct detection of the interaction of tiny analytes with receptors using an advanced plasmonic biosensor

F. C. Chien*, K. T. Huang, C. Y. Lin, S. J. Chen

*Corresponding author for this work

Research output: Contribution to journalConference article

1 Scopus citations

Abstract

The interaction between tiny-molecular-weight analytes and receptors can be detected directly using advanced plasmonic biosensors. This paper proposes a plasmonic biosensor with Au nanoclusters embedded in a dielectric film. The sensor uses the attenuated total reflection (ATR) method to excite both the surface plasmons (SPs) and the particle plasmons (PPs). The local electro-magnetic (EM) field is enhanced by controlling the size and volume fraction of the embedded Au nanoparticles. The developed biosensor demonstrates a 10-fold improvement in resolution compared to a conventional surface plasmon resonance (SPR) biosensor. Using the proposed ultra-sensitive plasmonic biosensor, a direct detection approach is adopted to analyze the interactions of tiny molecules (< 200 Da) without the need for high molecular weight competitors or explicit labeling. The plasmonic biosensor developed in this paper provides a rapid diagnostic capability and has considerable potential for use in pharmaceutical research.

Original languageEnglish
Article number19
Pages (from-to)107-117
Number of pages11
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5703
DOIs
StatePublished - 16 Aug 2005
EventPlasmonics in Biology and Medicine II - San Jose, CA, United States
Duration: 24 Jan 200525 Jan 2005

Keywords

  • Biomolecular interaction analysis
  • Direct detection
  • Plasmonic biosensors
  • Tiny analytes

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