Direct detection of C-reactive proteins in human serum using nanoparticle-enhanced surface plasmon resonance biosensing

H. Y. Lin*, K. Y. Tseng, W. P. Hu, H. Y. Hsu, A. Chiou, G. L. Chang, Shean-Jen Chen

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

C-reactive protein (CRP) produced by the liver is one of the most characteristic acute-phase proteins. It has been suggested that the level of CRP in human serum may be a significant tool of detecting risks of developing cardiovascular disease and atherosclerosis. Here we propose an advanced plasmonic surface plasmon resonance (SPR) bioassay with Au nanoparticles embedded in the dielectric film that demonstrates a 10X improvement in resolution compared to the conventional SPR biosensor. The co-sputtered film was modified with (3-Aminopropyl)triethoxysilane to sequentially immobilize protein G, monoclonal anti-CRP antibody (C8), and human serum albumins (HSA). After blocked by ethanolamine, the sensor was used to detect CRP. Using this extremely sensitive biochip, the lowest reliable concentration of CRP without any exterior labeling is simplified to human physiological level. The novel assay has the latent capability of not only eliminating the disturbances coming from serum proteins resulting in false signals, but is also able to be applied in rapid and label-free clinical detections of CRP with large improved sensitivity.

Original languageEnglish
Title of host publicationPlasmonics
Subtitle of host publicationNanoimaging, Nanofabrication, and their Applications II
DOIs
StatePublished - 21 Nov 2006
EventPlasmonics: Nanoimaging, Nanofabrication, and their Applications II - San Diego, CA, United States
Duration: 16 Aug 200617 Aug 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6324
ISSN (Print)0277-786X

Conference

ConferencePlasmonics: Nanoimaging, Nanofabrication, and their Applications II
CountryUnited States
CitySan Diego, CA
Period16/08/0617/08/06

Keywords

  • Atherosclerosis
  • C-reactive protein
  • Cardiovascular disease
  • Monoclonal antibody
  • Surface plasmon resonance biosensor

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