Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

C. Y. Cheng*, E. Perevedentseva, J. S. Tu, P. H. Chung, C. L. Cheng, K. K. Liu, Jui-I Chao, P. H. Chen, Chia-Ching Chang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

This letter presents direct observation of growth hormone receptor in one single cancer cell using nanodiamond-growth hormone complex as a specific probe. The interaction of surface growth hormone receptor of A549 human lung epithelial cells with growth hormone was observed using nanodiamond's unique spectroscopic signal via confocal Raman mapping. The growth hormone molecules were covalent conjugated to 100 nm diameter carboxylated nanodiamonds, which can be recognized specifically by the growth hormone receptors of A549 cell. The Raman spectroscopic signal of diamond provides direct and in vitro observation of growth hormone receptors in physiology condition in a single cell level.

Original languageEnglish
Article number163903
JournalApplied Physics Letters
Volume90
Issue number16
DOIs
StatePublished - 16 Apr 2007

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