Diffuse reflectance spectroscopy detects increased hemoglobin concentration and decreased oxygenation during colon carcinogenesis from normal to malignant tumors

Hsing Wen Wang*, Jeng Kai Jiang, Chi Hung Lin, Jen Kou Lin, Guang Jie Huang, Jia Sin Yu

*Corresponding author for this work

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Blood content and tumor oxygen level are important biomarkers and prognostic indicators in patients with colorectal cancer (CRC). However, noninvasive measurements of both quantities in human colon are limited. In this study, we extracted the total hemoglobin concentration (THC) and oxygen saturation (StO2) of normal, premalignant, and malignant colonic tissues in 27 patients using a diffuse reflectance instrument and algorithms based on the diffusion equation. The mean±standard error of THC and StO2 from all normal sites (n=26) is 93.4±17.1 μM and 67.2±3.7%, respectively. THC increased to 136.9±23.8μM and 153.8±38.6μM and StO2 decreased to 51.3±7.0% and 26.4±6.1% for premalignant and malignant tissues, respectively. The disease-to-normal THC ratios are 3.2±1.1 and 4.4±1.9 and the disease-to-normal StO2 ratios are 0.7±0.1 and 0.5±0.1 for premalignant and malignant tissues, respectively. These results demonstrate the feasibility of a robust optical method to assess colon THC and StO 2 at all stages of carcinogenesis in vivo so that the angiogenesis and hypoxia of the disease and the therapeutic role can be studied in CRC patients.

Original languageEnglish
Pages (from-to)2805-2817
Number of pages13
JournalOptics Express
Volume17
Issue number4
DOIs
StatePublished - 16 Feb 2009

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