Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array

T. Y. Liu; Y. Chen; H. H. Wang; Y. L. Huang; Y. C. Chao; K. T. Tsai; W. C. Cheng; C. Y. Chuang; Y. H. Tsai; C. Y. Huang; D. W. Wang; C. H. Lin; J. K. Wang; Y. L. Wang

doi:10.1166/jnn.2012.4941

Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array

T. Y. Liu, Y. Chen, H. H. Wang, Y. L. Huang, Y. C. Chao, K. T. Tsai, W. C. Cheng, C. Y. Chuang, Y. H. Tsai, C. Y. Huang, D. W. Wang, C. H. Lin, J. K. Wang, Y. L. Wang^*

^*Corresponding author for this work

Department of Biological Science and Technology

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23 Scopus citations

Abstract

We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes.

Original language	English
Pages (from-to)	5004-5008
Number of pages	5
Journal	Journal of Nanoscience and Nanotechnology
Volume	12
Issue number	6
DOIs	https://doi.org/10.1166/jnn.2012.4941
State	Published - 2012

Keywords

Bacteria
Classification
Nanoparticle Array
Surface-Enhanced Raman Scattering (SERS)

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Liu, T. Y., Chen, Y., Wang, H. H., Huang, Y. L., Chao, Y. C., Tsai, K. T., Cheng, W. C., Chuang, C. Y., Tsai, Y. H., Huang, C. Y., Wang, D. W., Lin, C. H., Wang, J. K., & Wang, Y. L. (2012). Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array. Journal of Nanoscience and Nanotechnology, 12(6), 5004-5008. https://doi.org/10.1166/jnn.2012.4941

Liu, T. Y. ; Chen, Y. ; Wang, H. H. ; Huang, Y. L. ; Chao, Y. C. ; Tsai, K. T. ; Cheng, W. C. ; Chuang, C. Y. ; Tsai, Y. H. ; Huang, C. Y. ; Wang, D. W. ; Lin, C. H. ; Wang, J. K. ; Wang, Y. L. / Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array. In: Journal of Nanoscience and Nanotechnology. 2012 ; Vol. 12, No. 6. pp. 5004-5008.

@article{6d5177a18c77436296875800b3840978,

title = "Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array",

abstract = "We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes.",

keywords = "Bacteria, Classification, Nanoparticle Array, Surface-Enhanced Raman Scattering (SERS)",

author = "Liu, {T. Y.} and Y. Chen and Wang, {H. H.} and Huang, {Y. L.} and Chao, {Y. C.} and Tsai, {K. T.} and Cheng, {W. C.} and Chuang, {C. Y.} and Tsai, {Y. H.} and Huang, {C. Y.} and Wang, {D. W.} and Lin, {C. H.} and Wang, {J. K.} and Wang, {Y. L.}",

year = "2012",

doi = "10.1166/jnn.2012.4941",

language = "English",

volume = "12",

pages = "5004--5008",

journal = "Journal of Nanoscience and Nanotechnology",

issn = "1533-4880",

publisher = "American Scientific Publishers",

number = "6",

}

Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array. / Liu, T. Y.; Chen, Y.; Wang, H. H.; Huang, Y. L.; Chao, Y. C.; Tsai, K. T.; Cheng, W. C.; Chuang, C. Y.; Tsai, Y. H.; Huang, C. Y.; Wang, D. W.; Lin, C. H.; Wang, J. K.; Wang, Y. L.

In: Journal of Nanoscience and Nanotechnology, Vol. 12, No. 6, 2012, p. 5004-5008.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array

AU - Liu, T. Y.

AU - Chen, Y.

AU - Wang, H. H.

AU - Huang, Y. L.

AU - Chao, Y. C.

AU - Tsai, K. T.

AU - Cheng, W. C.

AU - Chuang, C. Y.

AU - Tsai, Y. H.

AU - Huang, C. Y.

AU - Wang, D. W.

AU - Lin, C. H.

AU - Wang, J. K.

AU - Wang, Y. L.

PY - 2012

Y1 - 2012

N2 - We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes.

AB - We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes.

KW - Bacteria

KW - Classification

KW - Nanoparticle Array

KW - Surface-Enhanced Raman Scattering (SERS)

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U2 - 10.1166/jnn.2012.4941

DO - 10.1166/jnn.2012.4941

M3 - Article

C2 - 22905567

AN - SCOPUS:84863933992

VL - 12

SP - 5004

EP - 5008

JO - Journal of Nanoscience and Nanotechnology

JF - Journal of Nanoscience and Nanotechnology

SN - 1533-4880

IS - 6

ER -

Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array

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