Differentiation between normal and cancer cells with autofluorescence lifetime microscopy and nanosecond pulsed electric field effects

Nobuhiro Ohta, Kamlesh Awasthi, Hsin-Yun Hsu, Pei Ying Hsieh

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Fluorescence lifetime images of the endogenous nicotinamide adenine dinucleotide (NADH), which is well known as autofluorescence chromophores, were obtained for rat normal fibroblast cells (WFB) and H-ras oncogene-transfected WFB cancer cells (W31) and for human normal lung fibroblast (MRC-5) and human lung large carcinoma (HCI-H661). In both cases, the average lifetime of the NADH autofluorescence was shorter in cancer cells than in normal cells, indicating that the difference in metabolism between healthy and cancer cells might alter the level of coenzymes such as NADH. It is also shown that application of nanosecond pulsed electric field (nsPEF) induces apoptosis in these cell, resulting in the morphological changes and lengthening the autofluorescence lifetime of NADH. Furthermore, we found that nsPEF more efficiently affected cancer cells than normal cells in cell viability, suggesting the possibility of drug-free cancer therapy by nsPEF.

Original languageEnglish
Title of host publicationLabel-Free Biomedical Imaging and Sensing, LBIS 2019
EditorsNatan T. Shaked, Oliver Hayden
PublisherSPIE
ISBN (Electronic)9781510624221
DOIs
StatePublished - 4 Mar 2019
EventLabel-Free Biomedical Imaging and Sensing 2019, LBIS 2019 - San Francisco, United States
Duration: 2 Feb 20195 Feb 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10890
ISSN (Print)1605-7422

Conference

ConferenceLabel-Free Biomedical Imaging and Sensing 2019, LBIS 2019
CountryUnited States
CitySan Francisco
Period2/02/195/02/19

Keywords

  • Cancer and normal cells
  • Endogenous fluorophore
  • Fluorescence lifetime microscopy (FLIM)
  • Nanosecond pulsed electric field effect
  • Nicotinamide adenine dinucleotide

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