Differential in situ sensing of extra- and intracellular glutathione by a novel redox-responsive silica matrix-Au nanoprobe

Yun Ling Luo, Xin Chun Huang, Wei Ming Tu, Hsin-Yun Hsu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We synthesized a biothiol-sensitive nanoprobe by assembling gold nanoparticles with a novel redox-responsive silica (ReSi) matrix using dithiobis (succinimidyl propionate) and (3-aminopropyl) trimethoxysilane. Thin layer disulfide-bonded networks of the ReSi could differentially respond to extra- and intracellular glutathione in cancer cells within 30 min; furthermore, targeted cellular uptake could be monitored in situ by fluorescence recovery. Sigmoidal dose-response pattern of the nanoprobes presented in this study were attributed to the buried disulfide-linked 3D nanostructure of the ReSi nanoshell, optimized at an appropriate thickness, enabling not only buffering of small redox disturbances in the extracellular milieu but also the satisfied sensitivity for rapid redox sensing. Such a ReSi-functionalized gold nanoparticle-based nanoconjugate possesses the potential to serve as an effective intracellular drug carrier for future cancer theranostics.

Original languageEnglish
Pages (from-to)196-204
Number of pages9
JournalAnalytica Chimica Acta
Volume902
DOIs
StatePublished - 1 Jan 2016

Keywords

  • Cancer bio-sensing
  • Glutathione
  • Gold nanoparticle
  • Redox-responsive silica matrix

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