Development of microbead-based affinity biosensor by insulator-based dielectrophoresis

Tsung Min Chuo*, Wen-Syang Hsu, Shih Kang Fan

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This research describes a high sensitivity microfluidic bead-based immunosensor based on the principle of insulator-based dielectrophoresis (iDEP). An insulator film with small holes between two electrodes creates a nonuniform electric field. By applying appropriate voltage and frequency, the fluorescent beads are concentrated to lower electric field regions due to the difference of dielectric properties. This concentrating step enhances the fluorescence intensity of analytes and decreases the detection limit of immunosenser. In this research, the fluorescence dye is conjugated with streptavidin which has high affinity to biotin. We use biotin-labeled polystyrene beads to bind with streptavidin, therefore, we can further detect fluorescent streptavidin conjugates by a fluorescence microscope. The biotin-labeled polystyrene beads perform not only various chemical characteristics by labeling different functional groups but also offer an increased surface area for antibodies or antigens to immobilize on. Finally, we fabricate a microfluidic bead-based immunosensor with high sensitivity (1 pg/ml), short analysis time (∼10 minutes), few sample consumption (∼0.5 μl) and without physical microchannel.

Original languageEnglish
Title of host publication2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages187-190
Number of pages4
DOIs
StatePublished - 1 Jun 2012
Event7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan
Duration: 5 Mar 20128 Mar 2012

Publication series

Name2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Conference

Conference7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
CountryJapan
CityKyoto
Period5/03/128/03/12

Keywords

  • fluorescence
  • iDEP
  • immunosensor

Fingerprint Dive into the research topics of 'Development of microbead-based affinity biosensor by insulator-based dielectrophoresis'. Together they form a unique fingerprint.

  • Cite this

    Chuo, T. M., Hsu, W-S., & Fan, S. K. (2012). Development of microbead-based affinity biosensor by insulator-based dielectrophoresis. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 187-190). [6196753] (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196753