Determination of puerarin, daidzein, paeoniflorin, cinnamic acid, glycyrrhizin, ephedrine, and [6]-gingerol in Ge-gen-tang by micellar electrokinetic chromatography

Hsi Ya Huang, You-Zung Hsieh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

This paper presents a micellar electrokinetic chromatography (MEKC) method with diode-array detection to analyze for seven major components in the traditional Chinese medicinal preparation of Ge-gen-tang. Those analytes were successfully separated within 9 min using a pH 9.0 borate buffer containing 20 mM Sodium dodecyl sulfate at 30°C. Experimental results indicated that the relative standard deviations of the analytes' migration times were <0.43% and the correlation coefficients of the analytes' linear calibration graphs exceeded 0.996. The quantities of the seven components in four different Ge-gen-tang samples were determined by the MEKC method coupled with a relatively simple extraction method.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalAnalytica Chimica Acta
Volume351
Issue number1-3
DOIs
StatePublished - 30 Sep 1997

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