Determination of melamine in rat plasma, liver, kidney, spleen, bladder and brain by liquid chromatography-tandem mass spectrometry

Yu Tse Wu, Chih Min Huang, Chia Chun Lin, Wei An Ho, Lie Chwen Lin, Ting Fang Chiu, Der Cherng Tarng, Chi Hung Lin, Tung Hu Tsai*

*Corresponding author for this work

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

In this study, we describe a method for the analysis of melamine in rat plasma, liver, kidney, spleen, bladder, and brain using trichloroacetic acid precipitation with mixed-mode cation-exchange solid-phase extraction and hydrophilic interaction chromatography coupled to tandem mass spectrometry detection. Method validation was investigated completely, including linearity, precision, accuracy, matrix effect, extraction recovery, and carryover for the determination of melamine. The method exhibited a good linear range covering 20-500 ng/mL, and the overall precision ranged from 1.6 to 16.3%, with the accuracy varying from -7.9 to 15.1%. The mean matrix effects of melamine in rat plasma, liver, kidney, spleen, bladder, and brain ranged from 66.2 ± 6.7 to 95.5 ± 13.2%, and the mean recoveries for melamine varied from 79.8 ± 8.2 to 113.0 ± 9.6%. Rat kidney showed the highest level among the organs (192.5% of the plasma melamine level), and the average concentration of melamine in the brain was only 7.5% of the plasma melamine concentration. This work has pointed out that even with the application of two popular preparation procedures (acid precipitation and solid-phase extraction) of melamine, the matrix effect in analyzing biological samples still exists in certain kinds of matrices.

Original languageEnglish
Pages (from-to)7595-7601
Number of pages7
JournalJournal of Chromatography A
Volume1216
Issue number44
DOIs
StatePublished - 30 Oct 2009

Keywords

  • Acid precipitation
  • Carryover
  • Matrix effect
  • Melamine
  • Solid-phase extraction

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