Determination of cinnamaldehyde, cinnamic acid, paeoniflorin, glycyrrhizin and [6]-gingerol in the traditional Chinese medicinal preparation Kuei-chih-tang by cyclodextrin-modified micellar electrokinetic chromatography

Hsi Ya Huang, Kuang Lung Kuo, You-Zung Hsieh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

This study presents a cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique with a multi-wavelength detection method to determine specific components in Kuei-chih-tang. Kuei-chih-tang, a Chinese medicinal preparation, is composed of five crude herbs, i.e., Cinnamomi ramulus, Paeoniae radix, Glycyrrhizae radix, Zingiberis rhizoma and Zizyphi fructus. The effects of SDS and γ-cyclodextrin on the analytes' migration behavior were examined. The selected components were successfully separated within 10 min using a pH 10.0 borax-NaOH buffer containing 20 mM SDS and 2 mM γ-cyclodextrin. The correlation coefficients of the linear calibration graphs for the analytes exceeded 0.993. Moreover, the optimized CD-MEKC method was employed to analyze four different Kuei-chih-tang samples.

Original languageEnglish
Pages (from-to)267-274
Number of pages8
JournalJournal of Chromatography A
Volume771
Issue number1-2
DOIs
StatePublished - 30 May 1997

Keywords

  • Cinnamaldehyde
  • Cinnamic acid
  • Gingerol
  • Glycyrrhizin
  • Kuei-chih-tang
  • Paeoniflorin
  • Pharmaceutical analysis

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