Detection of Multiple Embryo Growth Factors by Bead-Based Digital Microfluidic Chip in Embryo Culture Medium

Chia Tung Lee*, Hsueh Yang Tseng, Yi Ting Jiang, Cheng Yeh Haung, Meng Shiue Lee, Wen-Syang Hsu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In IVF treatment, the pregnancy rate of selective single embryo implantation can be improved by detection the concentration of embryo growth factors such as IL -1β and TNF-α in embryo culture medium. In this study, we manufacture the high dielectric constant digital microfluidic chip and develop a Bead-based digital microfluidic immunoassay with low sample requirement, low limit of detection and rapid immunoassay. We establish the mouse TNF -α calibration curve and the requirement of sample is 520 nL, limit of detection is 1.55 pg/mL and total immunoassay time is 27 min. We also establish the Human IL - 1β calibration curve and the requirement of sample is 520 nL, limit of detection is 0.0579 pg/mL and total immunoassay time is 21 min.

Original languageEnglish
Title of host publicationNEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages119-122
Number of pages4
ISBN (Electronic)9781538652732
DOIs
StatePublished - 3 Dec 2018
Event13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2018 - Singapore, Singapore
Duration: 22 Apr 201826 Apr 2018

Publication series

NameNEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Conference

Conference13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2018
CountrySingapore
CitySingapore
Period22/04/1826/04/18

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  • Cite this

    Lee, C. T., Tseng, H. Y., Jiang, Y. T., Haung, C. Y., Lee, M. S., & Hsu, W-S. (2018). Detection of Multiple Embryo Growth Factors by Bead-Based Digital Microfluidic Chip in Embryo Culture Medium. In NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 119-122). [8556863] (NEMS 2018 - 13th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2018.8556863