Deleterious Effects of Arsenic, Benomyl and Carbendazim on Human Endometrial Cell Proliferation In Vitro

Chi Chen Chang, Yao Yuan Hsieh, Kung Hao Hsu, Horng Der Tsai, Wen Hsin Lin, Chih-Sheng Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Objective: We aimed to investigate the effects of arsenic (As), benomyl (Ben), and carbendazim (Carb) on endometrial cells. Materials and Methods: Human endometrial cells were obtained during diagnostic curettage. All cultured endometrial cells were divided into four groups: (1) 0 M (controls), (2) 10-6 M, (3) 10-5 M, (4) 10-4 M for As, Ben and Carb. After 24 and 48 hours in culture, endometrial cell proliferations were assessed by diphenyltetrazolium bromide assay. The influences of different concentrations of As, Ben and Carb upon the endometrium were compared. Results: During the first 24 hours, As, Ben and Carb appeared to have insignificant influences upon endometrial growth. After 48 hours in culture, all three agents significantly inhibited endometrial growth. In As groups, cell absorption after 48 hours culture were 100% (group 1), 82.1% (group 2), 43.6% (group 3) and 35.3% (group 4). In Ben groups, cell absorption was 100% (1), 75.9% (2), 66.4% (3) and 49. 6% (4). In the Carb groups, cell absorption was 100% (1), 70.4% (2), 73.0% (3) and 76.7% (4). Conclusion: The agents As, Ben and Carb appear to have inhibitory effects upon endometrial cells after 48 hours in culture.

Original languageEnglish
Pages (from-to)449-454
Number of pages6
JournalTaiwanese Journal of Obstetrics and Gynecology
Volume49
Issue number4
DOIs
StatePublished - 1 Dec 2010

Keywords

  • Arsenic
  • Benomyl
  • Carbendazim
  • Endometrium

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