CPred: A web server for predicting viable circular permutations in proteins

Wei-Cheng Lo, Li Fen Wang, Yen Yi Liu, Tian Dai, Jenn Kang Hwang, Ping Chiang Lyu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Circular permutation (CP) is a protein structural rearrangement phenomenon, through which nature allows structural homologs to have different locations of termini and thus varied activities, stabilities and functional properties. It can be applied in many fields of protein research and bioengineering. The limitation of applying CP lies in its technical complexity, high cost and uncertainty of the viability of the resulting protein variants. Not every position in a protein can be used to create a viable circular permutant, but there is still a lack of practical computational tools for evaluating the positional feasibility of CP before costly experiments are carried out. We have previously designed a comprehensive method for predicting viable CP cleavage sites in proteins. In this work, we implement that method into an efficient and user-friendly web server named CPred (CP site predictor), which is supposed to be helpful to promote fundamental researches and biotechnological applications of CP. The CPred is accessible at http://sarst.life.nthu.edu.tw/CPred.

Original languageEnglish
Pages (from-to)W232-W237
Number of pages6
JournalNucleic Acids Research
Issue numberW1
StatePublished - 1 Jul 2012

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