Core-shell magnetic nanoparticles of heparin conjugate as recycling anticoagulants

Ting Yu Liu, Li Ying Huang, Shang Hsiu Hu, Ming Chien Yang, San-Yuan Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Polyvinyl alcohol-shell magnetic nanoparticles (PVA-shell MNPs, PMNPs) were successfully prepared by in-situ co-precipitation process. Heparin (HEP) was then covalently conjugated on the PMNPs by using aminotrimethoxysilane (ATMS) and 4,4-diphenyl methane diisocyanate (HMDI) as molecular coupling agent (spacer). The morphology of the core-shell PMNPs was examined using transmission electron microscope (TEM), X-ray diffraction (XRD), and Raman spectrometry to demonstrate the core-shell structure, as well as X-ray photoelectron spectroscope (XPS) to provide direct evidence that the heparin molecules were immobilized on the surface of the core-shell PMNPs. Anticoagulant activity was evaluated with several parameters including activated partial thrombin time (APTT), prothrombin time (PT), fibrinogen time (FT), and thrombin time (TT). The results show that longer spacer would give better anticoagulant activity since lower steric hindrance can induce higher heparin grafting density. It might be anticipated that PMNPs of heparin conjugate can be used as anticoagulants instead of traditional free heparin, owing to its recycling characteristics by magnetic fields.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalJournal of Biomedical Nanotechnology
Issue number4
StatePublished - 1 Dec 2007


  • Anticoagulants
  • Core-shell
  • Heparin
  • Magnetic nanoparticles

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