Cooperative model for the binding of Xenopus transcription factor A to the 5S RNA gene

J. S. Hanas, D. F. Bogenhagen, Cheng-Wen Wu

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

78 particles containing 5S RNA and the transcription regulatory protein factor A have been purified to near homogeneity from Xenopus laevis oocytes. The binding of the transcription factor to the Xenopus borealis somatic 5S RNA gene has been monitored by quantitating the DNase I protection patterns of the protein-DNA interaction. Under stoichiometric binding conditions - i.e., when all added DNA binds to the factor - two protein molecules are required to saturate the 5S RNA gene. Under equilibrium binding conditions, titration of the 5S RNA gene with factor A results in a sigmoidal binding isotherm suggesting a cooperative interaction; half-saturation of binding is observed at a free factor A concentration of 1 nM. Cooperative binding between factor A and the 5S RNA gene may contribute to the stability of the transcription apparatus and its maintenance during cell division.

Original languageEnglish
Pages (from-to)2142-2145
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number8 I
DOIs
StatePublished - 1 Jan 1983

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