Confocal microscopic study on fluorescence quenching dynamics of single latex beads in poly(vinyl alcohol) film

Rachel Méallet-Renault, Hiroyuki Yoshikawa, Yoshiaki Tamaki, Tsuyoshi Asahi, Robert B. Pansu, Hiroshi Masuhara

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Fluorescence decay curves of single fluorescent poly-styrene beads in poly(vinyl alcohol) (PVA) films are measured with a confocal microscope, and quenching dynamics of the single beads by adsorbed methylene blue (MB) is examined. The fluorescence lifetimes of the beads are identical, while the quenching efficiency differs from bead to bead. This is related with inhomogeneity of adsorbed MB on the beads in PVA films. Non- exponential fluorescence decays of single beads with MB confirm that fluorophores near the bead surface are quenched by adsorbed quencher while the inner part of the bead is not affected. The importance of single bead fluorescence measurement is discussed.

Original languageEnglish
Pages (from-to)772-777
Number of pages6
JournalPolymers for Advanced Technologies
Volume11
Issue number8-12
DOIs
StatePublished - 1 Aug 2000

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