Fluorescence decay curves of single fluorescent poly-styrene beads in poly(vinyl alcohol) (PVA) films are measured with a confocal microscope, and quenching dynamics of the single beads by adsorbed methylene blue (MB) is examined. The fluorescence lifetimes of the beads are identical, while the quenching efficiency differs from bead to bead. This is related with inhomogeneity of adsorbed MB on the beads in PVA films. Non- exponential fluorescence decays of single beads with MB confirm that fluorophores near the bead surface are quenched by adsorbed quencher while the inner part of the bead is not affected. The importance of single bead fluorescence measurement is discussed.
|Number of pages||6|
|Journal||Polymers for Advanced Technologies|
|State||Published - 1 Aug 2000|