Computational prediction of new intein split sites

Yi Zong Lee, Wei-Cheng Lo*, Shih Che Sue

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Split inteins have emerged as a powerful tool in protein engineering. We describe a reliable in silico method to predict viable split sites for the design of new split inteins. A computational circular permutation (CP) prediction method facilitates the search for internal permissive sites to create artificial circular permutants. In this procedure, the original amino-and carboxyl-termini are connected and new termini are created. The identified new terminal sites are promising candidates for the generation of new split sites with the backbone opening being tolerated by the structural scaffold. Here we show how to integrate the online usage of the CP predictor, CPred, in the search of new split intein sites.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-268
Number of pages10
DOIs
StatePublished - 1 Jan 2017

Publication series

NameMethods in Molecular Biology
Volume1495
ISSN (Print)1064-3745

Keywords

  • Circular permutation
  • Protein ligation
  • Protein splicing
  • Split intein

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    Lee, Y. Z., Lo, W-C., & Sue, S. C. (2017). Computational prediction of new intein split sites. In Methods in Molecular Biology (pp. 259-268). (Methods in Molecular Biology; Vol. 1495). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6451-2_17