Comparative analysis of two UDP-glucose dehydrogenases in Pseudomonas aeruginosa PAO1

Ruei Jiun Hung, Han Sheng Chien, Ruei Zeng Lin, Ching Ting Lin, Jaya Vatsyayan, Hwei-Ling Peng, Hwan You Chang*

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

UDP-glucose dehydrogenase (UGDH) catalyzes a two-step NAD +-dependent oxidation of UDP-glucose to produce UDP-glucuronic acid, which is a common substrate for the biosynthesis of exopolysaccharide. Searching the Pseudomonas aeruginosa PAO1 genome data base for a UGDH has helped identify two open reading frames, PA2022 and PA3559, which may encode a UGDH. To elucidate their enzymatic identity, the two genes were cloned and overexpressed in Escherichia coli, and the recombinant proteins were purified. Both the gene products are active as dimers and are capable of utilizing UDP-glucose as a substrate to generate UDP-glucuronic acid. The Km values of PA2022 and PA3559 for UDP-glucose are ∼0.1 and 0.4 mM, whereas the Km values for NAD+ are 0.5 and 2.0 mM, respectively. Compared with PA3559, PA2022 exhibits broader substrate specificity, utilizing TDP-glucose and UDP-N-acetylglucosamine with one-third the velocity of that with UDP-glucose. The PA2022 mutant and PA2022-PA3559 double mutant, but not the PA3559 mutant, are more susceptible to chloramphenicol, cefotaxime, and ampicillin. The PA3559 mutant, however, shows a reduced resistance to polymyxin B compared with wild type PAO1. Finally, real time PCR analysis indicates that PA3559 is expressed primarily in low concentrations of Mg2+, which contrasts with the constitutive expression of PA2022. Although both the enzymes catalyze the same reaction, their enzymatic properties and gene expression profiles indicate that they play distinct physiological roles in P. aeruginosa, as reflected by different phenotypes displayed by the mutants.

Original languageEnglish
Pages (from-to)17738-17748
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number24
DOIs
StatePublished - 15 Jun 2007

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    Hung, R. J., Chien, H. S., Lin, R. Z., Lin, C. T., Vatsyayan, J., Peng, H-L., & Chang, H. Y. (2007). Comparative analysis of two UDP-glucose dehydrogenases in Pseudomonas aeruginosa PAO1. Journal of Biological Chemistry, 282(24), 17738-17748. https://doi.org/10.1074/jbc.M701824200