Coherent interaction of optical second harmonic generation in collagen fibrils

Shi Wei Chu*, Shih Peng Tai, Ming Che Chan, Chi Kuang Sun, I. Ching Hsiao, Chi Hung Lin, Yung Chih Chen, Bai Ling Lin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


We present the first experimental comparison between optical second harmonic generation images and atomic force microscope images in a matrix of nano-scaled collagen fibrils. Substantial variation of forward and backward propagated second harmonic generation radiation is observed in a single collagen fibril and is nicely correlated with the accurately determined thickness from an atomic force microscope. Contradicting to conventional nonlinear optical theory, our result indicates a linear relationship between fibril thickness and forward / backward second harmonic generation ratio. This is the first demonstration of estimating fibril thickness with nanometer precision by a noninvasive optical method.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences VIII
StatePublished - 21 Apr 2008
EventMultiphoton Microscopy in the Biomedical Sciences VIII - San Jose, CA, United States
Duration: 20 Jan 200822 Jan 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMultiphoton Microscopy in the Biomedical Sciences VIII
CountryUnited States
CitySan Jose, CA


  • Atomic force microscope
  • Nonlinear scattering
  • Optical interference
  • Radiation pattern
  • Three-dimensional microscopy
  • Type I collagen

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