Cloning, characterization and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus

Hwei-Ling Peng, R. P. Novick, B. Kreiswirth, J. Kornblum, P. Schlievert

Research output: Contribution to journalArticlepeer-review

387 Scopus citations

Abstract

We have previously identified a gene in Staphylococcus aureus, agr, whose activity is required for high-level post-exponential-phase expression of a series of secreted proteins. In this paper, we describe the cloning of this gene in Escherichia coli by using an inserted transposon (Tn551) as a cloning probe. The cloned gene, consisting of a 241-codon open reading frame containing the site of the transposon insertion, was recloned to an S. aureus vector, pSK265, and shown to be functional in S. aureus. Activity was evaluated by determinations of α-hemolysin, β-hemolysin, and toxic shock syndrome toxin-1 production in early-stationary-phase cultures. The cloned gene showed considerable variation with respect to different exoproteins and different host strains compared with the chromosomal agr determinant; this variation could not be attributed to the higher copy number of the cloned gene and probably reflects inapparent subtleties of the regulatory system.

Original languageEnglish
Pages (from-to)4365-4372
Number of pages8
JournalJournal of Bacteriology
Volume170
Issue number9
DOIs
StatePublished - 1 Jan 1988

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