Cloning and expression of the Klebsiella pneumoniae galactose operon

Hwei-Ling Peng, Tzu Fun Fu, Sou Feng Liu, Hwan You Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The entire galactose (gal) operon of Klebsiella pneumoniae was isolated and functionally analyzed in Escherichia coli The genes encoding galactokinase (galK), galactose-1-phosphate uridyltransferase (galT), and UDP-galactose-4-epimerase (galE) were mapped by complementation analysis. The gene order E-T-K was found to be identical to that of Salmonella spp. and E. coli. Analysis of the nucleotide sequence in the control region revealed significant homology with that of E. coli Two major sites for transcriptional initiation, both mapped to a cytosyl residue, were identified by primer extension. When the operon is expressed in E. coli, the K. pneumoniae gal gene products make up about 30% of the total cellular proteins. The presence of a powerful promoter responsible for high level synthesis of the gal proteins was also demonstrated using β-galactosidase as reporter.

Original languageEnglish
Pages (from-to)604-608
Number of pages5
JournalJournal of Biochemistry
Volume112
Issue number5
DOIs
StatePublished - 1 Jan 1992

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