Cloning and characterization of the endogenous retroviral-tRNAGlu multigene family from human genomes of different racial backgrounds

Kai Wun Yeh, Wen K. Yang, Huey Chung Huang, Ya Nan Feng, Jau Ching Liu, Felicia Y.H. Wu, Cheng-Wen Wu Lee*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

An 8.3-kb human endogenous retroviral-tRNAGlu (HERV-E)-encoding cDNA clone and a 1.5-kb genomic clone were isolated from a Chinese-derived cervical cancer cell line, CC7T, and their sequences determined. The former is a full-length endogenous retroviral cDNA containing corresponding u5-gag-pol-env-u3-r regions. The latter is a partial retroviral DNA segment, covering the gag and pol genes. Analysis of normal human DNA by Southern blot hybridization with three specific HERV-E molecular DNA probes revealed complex restriction-fragment length polymorphisms (RFLP), implying that the human genome contains diverse proviral structures and dispersed integration sites. The complex patterns were virtually identical between DNAs from African-Americans, Asians and Caucasians, with only a few minor variations. The data suggest that these proviral sequences were mostly incorporated into the human genome before racial divergence and, hence, may serve as markers for distinct chromosomal sites.

Original languageEnglish
Pages (from-to)247-252
Number of pages6
JournalGene
Volume155
Issue number2
DOIs
StatePublished - 3 Apr 1995

Keywords

  • Proviral structure
  • restriction-fragment length polymorphism
  • transduced sequence
  • truncated structure

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