Characterization of two populations of mesenchymal progenitor cells in umbilical cord blood

Yu Jen Chang, Ching-Ping Tseng, Lee Feng Hsu, Tzu Bou Hsieh, Shiaw Min Hwang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Umbilical cord blood (UCB) is a valuable source for hematopoietic progenitor cell therapy. Moreover, it contains another subset of non-hematopoietic population referred to as mesenchymal progenitor cells (MPCs), which can be ex vivo expanded and differentiated into osteoblasts, chondrocytes and adipocytes. In this study, we successfully isolated the clonogenic MPCs from UCB by limiting dilution method. These cells exhibited two different morphologic phenotypes, including flattened fibroblasts (majority) and spindle-shaped fibroblasts (minority). Both types of MPCs shared similar cell surface markers except CD90 and had similar osteogenic and chondrogenic potentials. However, the spindle-shaped clones possessed the positive CD90 expression and showed a greater tendency in adipogenesis, while the flattened clones were CD90 negative cells and showed a lower tendency in adipogenesis. The high number of flattened MPCs might be linked to the less sensitivity of UCB-derived MPCs in adipogenic differentiation.

Original languageEnglish
Pages (from-to)495-499
Number of pages5
JournalCell Biology International
Volume30
Issue number6
DOIs
StatePublished - 1 Jun 2006

Keywords

  • Clonogenic
  • Differentiation
  • Mesenchymal progenitor cells

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