Characterization of corneal damage from Pseudomonas aeruginosa infection by the use of multiphoton microscopy

Yu Lin Chang; Wei Liang Chen; Wen Lo; Shean-Jen Chen; Hsin Yuan Tan; Chen Yuan Dong

doi:10.1063/1.3505360

Characterization of corneal damage from Pseudomonas aeruginosa infection by the use of multiphoton microscopy

Yu Lin Chang, Wei Liang Chen, Wen Lo, Shean-Jen Chen, Hsin Yuan Tan^*, Chen Yuan Dong

^*Corresponding author for this work

Institute of Imaging and Biomedical Photonics

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Using multiphoton autofluorescence (MAF) and second harmonic generation (SHG) microscopy, we investigate the morphology and the structure of the corneal epithelium and stroma collagen of bovine cornea following injection of Pseudomonas aeruginosa. We found that corneal epithelial cells are damaged and stromal collagen becoming increasingly autofluorescent with time. We also characterized infected cornea cultured for 0, 6, 12, and 24 h by quantitative ratiometric MAF to SHG index (MAFSI) analysis. MAFSI results show that the destruction of the stromal collagen corresponds to a decrease in SHG intensity and increase of MAF signal with time.

Original language	English
Article number	183703
Journal	Applied Physics Letters
Volume	97
Issue number	18
DOIs	https://doi.org/10.1063/1.3505360
State	Published - 1 Nov 2010

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title = "Characterization of corneal damage from Pseudomonas aeruginosa infection by the use of multiphoton microscopy",

abstract = "Using multiphoton autofluorescence (MAF) and second harmonic generation (SHG) microscopy, we investigate the morphology and the structure of the corneal epithelium and stroma collagen of bovine cornea following injection of Pseudomonas aeruginosa. We found that corneal epithelial cells are damaged and stromal collagen becoming increasingly autofluorescent with time. We also characterized infected cornea cultured for 0, 6, 12, and 24 h by quantitative ratiometric MAF to SHG index (MAFSI) analysis. MAFSI results show that the destruction of the stromal collagen corresponds to a decrease in SHG intensity and increase of MAF signal with time.",

author = "Chang, {Yu Lin} and Chen, {Wei Liang} and Wen Lo and Shean-Jen Chen and Tan, {Hsin Yuan} and Dong, {Chen Yuan}",

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AU - Chen, Wei Liang

AU - Lo, Wen

AU - Chen, Shean-Jen

AU - Tan, Hsin Yuan

AU - Dong, Chen Yuan

PY - 2010/11/1

Y1 - 2010/11/1

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AB - Using multiphoton autofluorescence (MAF) and second harmonic generation (SHG) microscopy, we investigate the morphology and the structure of the corneal epithelium and stroma collagen of bovine cornea following injection of Pseudomonas aeruginosa. We found that corneal epithelial cells are damaged and stromal collagen becoming increasingly autofluorescent with time. We also characterized infected cornea cultured for 0, 6, 12, and 24 h by quantitative ratiometric MAF to SHG index (MAFSI) analysis. MAFSI results show that the destruction of the stromal collagen corresponds to a decrease in SHG intensity and increase of MAF signal with time.

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