Characterization of corneal damage from Pseudomonas aeruginosa infection by the use of multiphoton microscopy

Yu Lin Chang, Wei Liang Chen, Wen Lo, Shean-Jen Chen, Hsin Yuan Tan*, Chen Yuan Dong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Using multiphoton autofluorescence (MAF) and second harmonic generation (SHG) microscopy, we investigate the morphology and the structure of the corneal epithelium and stroma collagen of bovine cornea following injection of Pseudomonas aeruginosa. We found that corneal epithelial cells are damaged and stromal collagen becoming increasingly autofluorescent with time. We also characterized infected cornea cultured for 0, 6, 12, and 24 h by quantitative ratiometric MAF to SHG index (MAFSI) analysis. MAFSI results show that the destruction of the stromal collagen corresponds to a decrease in SHG intensity and increase of MAF signal with time.

Original languageEnglish
Article number183703
JournalApplied Physics Letters
Volume97
Issue number18
DOIs
StatePublished - 1 Nov 2010

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