Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Tzu Ying Li, Biing Hui Liu, Yu-Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

The intact fungal spores of several strains of four Aspergillus species, Aspergillus flavus, A. oryzae, A. parasiticus, and A. sojae, were directly analyzed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry. Very simple MALDI mass spectra are obtained by directly mixing spores with a matrix such as α-cyano-4-hydroxycinnamic acid or sinapinic acid. The mass spectra are obtained from the ablation of cell walls of spores owing to the acidity of the matrix solution. The MALDI results show that aflatoxigenic strains and non-aflatoxigenic strains have different mass peak profiles. Furthermore, the MALDI results of non-aflatoxigenic A. flavus and A. parasiticus spores resemble those of the closely related A. oryzae and A. sojae spores, respectively. (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish
Pages (from-to)2393-2400
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume14
Issue number24
DOIs
StatePublished - 1 Dec 2000

Fingerprint Dive into the research topics of 'Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry'. Together they form a unique fingerprint.

Cite this