Cell sweeper for microfluidic cavity

Chia-Hung Tsai*, Makoto Kaneko

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

A method to sweep out cells from an one-end-closed microfluidic cavity is presented with experimental results. Cell removal is useful for eliminating stuck cells or renewing a culture cavity. However, it cannot be easily achieved due to the flow limitation in a microfluidic system. The idea of the proposed method is to remove cells by secondary flow induced by periodic flow pumping. In experiment, a sequence of frequencies were applied to a cavity pre-filled with cells. We found secondary flow at 20Hz and 40Hz, and the flow effectively removed the cells in the cavity just within few seconds.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages1537-1538
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 1 Jan 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • Cell removal
  • Pdms microfluidics
  • Secondary flow

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