Cell compatible trimethoprim-decorated iron oxide nanoparticles bind dihydrofolate reductase for magnetica ly modulating focal adhesion of mammalian cells

Marcus J.C. Long, Yue Pan, Hsin-Chieh Lin, Lizbeth Hedstrom, Bing Xu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

On the basis of the high affinity binding of trimethoprim (TMP) to Escherichia coli dihydrofolate reductase (eDHFR), TMP-decorated iron oxide nanoparticles bind to eDHFR with high affinity and specificity, which allows magnetic modulation of focal adhesion of mammalian cells adhered to a surface. Besides being the first example of nanoparticles that selectively bind to eDHFR, the biocompatibility of the conjugate of TMP - iron oxide nanoparticles renders a convenient and versatile platform for investigating the cellular responses to specific, mechanical perturbation of proteins via a magnetic force.

Original languageEnglish
Pages (from-to)10006-10009
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number26
DOIs
StatePublished - 6 Jul 2011

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