Cartilage tissue-mimetic pellets with multifunctional magnetic hyaluronic acid-graft-amphiphilic gelatin microcapsules for chondrogenic stimulation

Kai Ting Hou, Ting Yu Liu, Min Yu Chiang, Chun Yu Chen, Shwu Jen Chang*, San Yuan Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Articular cartilage defect is a common disorder caused by sustained mechanical stress. Owing to its nature of avascular, cartilage had less reconstruction ability so there is always a need for other repair strategies. In this study, we proposed tissue-mimetic pellets composed of chondrocytes and hyaluronic acid-graft-amphiphilic gelatin microcapsules (HA-AGMCs) to serve as biomimetic chondrocyte extracellular matrix (ECM) environments. The multifunctional HA-AGMC with specific targeting on CD44 receptors provides excellent structural stability and demonstrates high cell viability even in the center of pellets after 14 days culture. Furthermore, with superparamagnetic iron oxide nanoparticles (SPIOs) in the microcapsule shell of HA-AGMCs, it not only showed sound cell guiding ability but also induced two physical stimulations of static magnetic field(S) and magnet-derived shear stress (MF) on chondrogenic regeneration. Cartilage tissue-specific gene expressions of Col II and SOX9 were upregulated in the present of HA-AGMC in the early stage, and HA-AGMC+MF+S held the highest chondrogenic commitments throughout the study. Additionally, cartilage tissue-mimetic pellets with magnetic stimulation can stimulate chondrogenesis and sGAG synthesis.

Original languageEnglish
Article number785
JournalPolymers
Volume12
Issue number4
DOIs
StatePublished - 1 Apr 2020

Keywords

  • Amphiphilic gelatin microcapsules
  • Cartilage tissue
  • CD44 receptor
  • Magnetic stimulation
  • Tissue-mimetic pellets

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