Carboxylate-functionalized iron oxide nanoparticles in surface-assisted laser desorption/ionization mass spectrometry for the analysis of small biomolecules

Yu Chih Chiu, Yu-Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Iron oxide particles have been demonstrated previously as an effective SALDI-assisted material in SALDI MS analysis. The addition of a proton source such as citrate salts is helpful for rendering ionization efficiency. To simplify the sample preparation steps, we immobilize polyacrylic acid (PAA) onto the surface of the iron oxide particles. The modified iron oxide particles can be used as the SALDI-assisted material without the addition of extra proton source. Small biomolecules including bradykinin, melittin, and insulin are used to examine the feasibility of this approach. The results show that the SALDI mass spectral results are improved by using polyacrylic acid (MWaverage= ∼2 kDa) coated iron oxide nanoparticles.

Original languageEnglish
Pages (from-to)260-267
Number of pages8
JournalAnalytical Letters
Volume41
Issue number2
DOIs
StatePublished - 1 Jan 2008

Keywords

  • Iron oxide
  • Nanoparticles
  • Polyacrylic acid
  • SALDI

Fingerprint Dive into the research topics of 'Carboxylate-functionalized iron oxide nanoparticles in surface-assisted laser desorption/ionization mass spectrometry for the analysis of small biomolecules'. Together they form a unique fingerprint.

Cite this