TY - JOUR
T1 - Carbon nanotubes as affinity probes for peptides and proteins in MALDI MS analysis
AU - Chen, Wei Yu
AU - Wang, Lung Shen
AU - Chiu, Hsin-Tien
AU - Chen, Yu-Chie
AU - Lee, Chi Young
PY - 2004/11/1
Y1 - 2004/11/1
N2 - Recently, carbon nanotubes (CNTs) have been reported to be an effective MALDI matrix for small molecules (Anal. Chem. 2003, 75, 6191). In a somewhat related study, we have employed CNTs produced by using NaH-treated anodic aluminum oxide (Na@AAO) as a reactive template as the assisting matrix for MALDI analysis upon the addition of high concentrations of citrate buffer. Our results indicate that the mass range can be extended to ca. 12,000 Da and that alkali metal adducts of analytes are effectively reduced. Furthermore, we have employed citric acid-treated CNTs as affinity probes to selectively concentrate traces of analytes from aqueous solutions. High concentrations of salts and surfactants in the sample solutions are also tolerated. This approach is very suitable for the MALDI analysis of small proteins, peptides, and protein enzymatic digest products.
AB - Recently, carbon nanotubes (CNTs) have been reported to be an effective MALDI matrix for small molecules (Anal. Chem. 2003, 75, 6191). In a somewhat related study, we have employed CNTs produced by using NaH-treated anodic aluminum oxide (Na@AAO) as a reactive template as the assisting matrix for MALDI analysis upon the addition of high concentrations of citrate buffer. Our results indicate that the mass range can be extended to ca. 12,000 Da and that alkali metal adducts of analytes are effectively reduced. Furthermore, we have employed citric acid-treated CNTs as affinity probes to selectively concentrate traces of analytes from aqueous solutions. High concentrations of salts and surfactants in the sample solutions are also tolerated. This approach is very suitable for the MALDI analysis of small proteins, peptides, and protein enzymatic digest products.
UR - http://www.scopus.com/inward/record.url?scp=7244255952&partnerID=8YFLogxK
U2 - 10.1016/j.jasms.2004.08.001
DO - 10.1016/j.jasms.2004.08.001
M3 - Article
C2 - 15519230
AN - SCOPUS:7244255952
VL - 15
SP - 1629
EP - 1635
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
SN - 1044-0305
IS - 11
ER -