Carbon dots prepared from ginger exhibiting efficient inhibition of human hepatocellular carcinoma cells

Chi Lin Li, Chung Mao Ou, Chih Ching Huang, Wei Cheng Wu, Yi Ping Chen, Tzu-En Lin, Lin Chen Ho, Chia Wei Wang, Chung Chien Shih, Hang Cheng Zhou, Ying Chu Lee, Woan Fang Tzeng, Tzeon Jye Chiou, Sin Tak Chu, Jinshun Cang, Huan Tsung Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

Fluorescent carbon nanodots (C-dots; 4.3 ± 0.8 nm) from fresh tender ginger juice provide high suppression of the growth of human hepatocellular carcinoma cells (HepG2), with low toxicity to normal mammary epithelial cells (MCF-10A) and normal liver cells (FL83B). The inhibition is selective to HepG2 over other tested cancer cells, including human lung cancer cell line (A549), human breast cancer cell line (MDA-MB-231), and human cervical cancer cell line (HeLa). Western blot results reveal that the C-dots up-regulate the expression of p53 protein only in the HepG2 cell line. The 50% inhibiting concentration (IC50) value of the C-dots on HepG2 cells is 0.35 mg mL-1. Image cytometry results show significant uptake of C-dots by HepG2 cells that induce intracellular production of reactive oxygen species (ROS, 18.2-fold increased), while other cells remain almost the same in ROS levels after treatment with C-dots (1.11 mg mL-1). The C-dots trigger the pro-apoptotic factor to promote HepG2 cell apoptosis. The C-dots effectively inhibit the growth of tumors in nude mice (104 ± 14 vs. 3.7 ± 0.2 mg with and without treatment within 14 days). This journal is

Original languageEnglish
Pages (from-to)4564-4571
Number of pages8
JournalJournal of Materials Chemistry B
Volume2
Issue number28
DOIs
StatePublished - 28 Jul 2014

Fingerprint Dive into the research topics of 'Carbon dots prepared from ginger exhibiting efficient inhibition of human hepatocellular carcinoma cells'. Together they form a unique fingerprint.

Cite this