Candida albicans ENO1 null mutants exhibit altered drug susceptibility, hyphal formation, and virulence

Hui-Ching Ko, Ting Yin Hsiao, Chiung-Tong Chen, Yun-Liang Yang

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Abstract

We previously showed that the expression of ENO1 (enolase) in the fungal pathogen Candida albicans is critical for cell growth. In this study, we investigate the contribution of the ENO1 gene to virulence. We conducted our functional study of ENO1 in C. albicans by constructing an eno1/eno1 null mutant strain in which both ENO1 alleles in the genome were knockouted with the SAT1 flipper cassette that contains the nourseothricin-resistance marker. Although the null mutant failed to grow on synthetic media containing glucose, it was capable of growth on media containing yeast extract, peptone, and non-fermentable carbon sources. The null mutant was more susceptible to certain antifungal drugs. It also exhibited defective hyphal formation, and was avirulent in BALB/c mice.
Original languageEnglish
Pages (from-to)345-351
Number of pages7
JournalJournal of Microbiology
Volume51
Issue number3
DOIs
StatePublished - Jun 2013

Keywords

  • enolase; opportunistic human pathogen; drug susceptibility; hyphal formation; virulence
  • CANDIDA-ALBICANS ENOLASE; CELL-WALL PROTEINS; SACCHAROMYCES-CEREVISIAE; SYSTEMIC CANDIDIASIS; GLYCOLYTIC ENZYME; MOLECULAR-CLONING; GENE-EXPRESSION; IDENTIFICATION; YEAST; MICE

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