Bead-based digital microfluidic immunoassay for IL-1β detection in embryo culture medium

Hsueh Yang Tseng, Yi Ting Jiang, Cheng Yeh Huang, Meng Shiue Lee, Wen-Syang Hsu, Da Jeng Yao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

One of the challenges in IVF treatment is to improve the pregnancy rate of selective single embryo implantation. The concentration of IL-1β in embryo culture medium may help to predict pregnancy rate. Clinically, each embryo can only get small amount of sample size. However, the traditional immunoassay cannot detect biomarkers in such small sample sizes. Therefore, we combined immunoassay with the bead-based digital microfluidic chip. The chip has two advantages, low sample requirement and low detection limit. We established the mouse IL-1β calibration curve, confirm the Human Tubal Fluid (HTF) does not influence the test result and demonstrate that mouse IL-1β in clinical mouse embryo culture medium is positively correlate to cultured days. This device has a great potential for increasing IVF pregnancy rates.

Original languageEnglish
Title of host publication2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages295-298
Number of pages4
ISBN (Electronic)9781509030590
DOIs
StatePublished - 25 Aug 2017
Event12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States
Duration: 9 Apr 201712 Apr 2017

Publication series

Name2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Conference

Conference12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
CountryUnited States
CityLos Angeles
Period9/04/1712/04/17

Keywords

  • digital microfluidic
  • embryo growth factor
  • immunoassay

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    Tseng, H. Y., Jiang, Y. T., Huang, C. Y., Lee, M. S., Hsu, W-S., & Yao, D. J. (2017). Bead-based digital microfluidic immunoassay for IL-1β detection in embryo culture medium. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 (pp. 295-298). [8017028] (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2017.8017028