Automatic method to compare the lanes in gel electrophoresis images

Chih Yang Lin*, Yu-Tai Ching, Yun Liang Yang

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Gel electrophoresis (GE) is an important tool in genomic analysis. GE results are presented using images. Each image contains several vertical lanes. Each lane consists of several horizontal bands. Two lanes are identical if the relative positions of the bands are the same. We present a computer method designed to compare the lanes and identify identical lanes. This method, developed using many image-processing techniques, is applied to segment the lanes and bands in GE images. The lanes are then converted into "position vectors" that describe the positions of the bands. Comparing lanes becomes equivalent to comparing the position vectors. This method can accurately identify identical lanes, helping biologists to identify the identical lanes from many lanes with much less effort.

Original languageEnglish
Pages (from-to)179-189
Number of pages11
JournalIEEE Transactions on Information Technology in Biomedicine
Volume11
Issue number2
DOIs
StatePublished - 1 Mar 2007

Keywords

  • Dynamic programming
  • Gel electrophoresis (GE)
  • Lane comparison
  • Matched filter
  • Segmentation
  • Watershed method

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